Earlier this month, Uber's new chief executive Dara Khosrowshahi met Mike Brown, who runs Transport for London.
An Uber spokeswoman said that today's filing in the Westminster Magistrates' Court is more a formal notification that the appeal is underway and that the company may never appear in court if a settlement is reached. Uber plans to put forward further reasons for its appeal before a planned hearing on December 11.
Uber's licence expired on 30 September after it was told by TfL told it that it would not be renewed over concerns that the ride-hailing firm is not "fit and proper" to operate a private hire service in London, citing concerns with the "conduct and approach" of the firm.
Transport for London (TfL) claims that the conduct of Uber, which has about 40,000 drivers and 3.5 million customers in the British capital, had raised concerns.
Its spokesman added that details of Uber's arguments against the decision will not be released until nearer the time of the appeal being heard. And tweeted to urge London to work with the company "to make things right".
"While Uber has revolutionised the way people move in cities around the world, it's equally true that we've got things wrong along the way", he said.
Mayor Sadiq Khan support TfL's decision and said: "All companies in London must play by the rules, if you do play by the rules, you're welcome in London".
Mr Khosrowshahi took over at Uber in August after predecessor Travis Kalanick resigned following a series of scandals.
But Uber has seen also its global popularity explode since it launched in 2009 in San Francisco.