The British quartet of CJ Ujah, Adam Gemili, Danny Talbot and Nethaneel Mitchell-Blake pipped the United States to gold in a world-leading 37.47 seconds, but only after Bolt had pulled up on his anchor leg and collapsed to the track.
"Usain was getting cold and he said "I don't like this".
Though not in the manner he would have planned or thought about, Usain Bolt says final goodbye to Team Jamaica and extends love for fans after seeing curtains being drawn to his illustrious track & field career in a rather disheartening manner. "40 minutes and two medal presentations before our run".
But after tonight's triumph, he said: "2012 didn't go our way and we have been working hard since then". And to see a true legend, a true champion go out there and struggling like that.
"It was ridiculous. We were there waiting, and we were really trying to stay warm, but it was really ridiculous, we waited a really long time", McLeod said, via Jamaica-Gleaner. But it was ridiculous. "Now he's really deserved time away and to not have to deal with the gruelling training".
Justin Gatlin who has been banned twice for using a banned substance shocked the whole world as he won the 100m race ending Usain Bolt's reign at the top in his final game on track.
"He didn't tell us exactly what happened but from what I saw, it looked like a strain or a cramp of some sort", Forte said.
Despite a disappointing end to his career, Bolt retires with an incredible haul of 19 major gold medals. I think it got the better of us.
Bolt's teammates complained that his problem was caused by organizers keeping the relay teams waiting in the cold before their race as several medal ceremonies were held.
While Gatlin and the other Team USA sprinters, including Mike Rodgers, Jaylen Bacon and 100-meter silver medalist Christian Coleman, appeared disappointed with second place, it sure beats last summer's Olympic performance in which Team USA was disqualified for mishandling the baton.
"It's a cramp in his left hamstring, but a lot of the pain is from disappointment from losing the race", Jamaican team doctor Kevin Jones said.
No matter the result of the relay, organisers said Bolt will be afforded a dedicated lap of honour on Sunday in tribute to his incredible track career.
"Bolt is an electrifying character who has run sizzling times, mind-blowing times and throughout the years he has always kept it classy".