The team practicing in full pads just over 36 hours after their first pre-season game.
Wolfe was clearly distraught as he was getting carted off the field.
Wolfe's injury is the latest on the Broncos' defensive front, coming on the same day Billy Winn was placed on injured reserve after tearing his right ACL in Denver's preseason opener at Chicago on Thursday night.
Wolfe could not put any weight on the leg as he was helped inside the Broncos' complex. Winn's injury created a path for Shebly Harris to make the team.
Fortunately for the Broncos, it looks like Wolfe won't miss an extended period of time, although that has yet to be confirmed.
Wolfe had 51 tackles and 5.5 sacks previous year and is a huge part of the Broncos run defense and pass rush. Vance Walker remains available.
The Broncos' defense is already without starting outside linebacker Shane Ray for one or two regular season games with a torn right wrist ligament. He missed all of last season with a knee injury suffered in training camp last season while going against Russell Okung.
Denver Broncos running back C.J. Anderson. Other rotational and depth players at defensive end include Zach Kerr, Adam Gostis, Harris and Shakir Soto. Nose tackle Kyle Peko (foot) is yet to return from the physically unable to perform list.